HPlC Analysis of Phospholipids by Evaporative laser light-Scattering Detection
نویسندگان
چکیده
oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture content. Anal· ytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary column chromatography for fatty acid composition, and by reverse-phase C-1S HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine.
منابع مشابه
Detectors for High-performance Liquid Chromatography of Lipids with Special Reference to Evaporative Light-scattering Detectio
A. Introduction B. Optical and Spectrophotometric Detectors 1. Differential refractometry 2. Ultraviolet spectrophotometry 3. Fluorescence detection 4. Infrared spectrophotometric detectors 5. Spectrophotometric detection with post-column chemical reaction C. Some Miscellaneous Detection Systems 1. The mass spectrometer as an HPLC detector 2. Radioactivity detectors 3. Density, electrochemical ...
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